NUIG Flow Cytometry Core Facility


Background

The facility was established in June 2011 through funding obtained from Science Foundation Ireland (SFI), Higher Education Authority (HEA), Research Support Fund 2011 and the appointment of Programme for Research in Third Level Institutions Cycle 5 (PRTLI 5) funded fulltime dedicated Senior Technical Officer.

Mission Statement

To establish a flow cytometry centre of excellence which will provide a high quality service, modelled on international best practice, to the NUIG research community and industrial customers.

Technology

Flow cytometry is a multi-parametric technology that measures fluorescent signals and laser light scatter produced by cells or particles as they pass or flow through a laser light source. These signals can be correlated to structural and/or functional cell parameters.
 
Highly specialised flow cytometers, cell sorters, can physically separate or sort defined subpopulations of cells from a heterogeneous mixture.
 

Flow cytometry is an essential tool for basic and translational biomedical research, is commonly used in clinical environments and is rapidly increasing in its range of industrial applications.


Equipment
 
     Technical Resources
 
 
     
  • 4 laser, 12 parameter Merck Imagestream Mark II imaging flow cytometer
 
 
 
  •  Class II Biological Safety Cabinet
 
 
 
        Equipment Booking
 
  Equipment can be booked online via the NCBES intranet.
 
 
 
       Equipment Usage Cost 2017
 
  • BD Accuri C6 Sampler €15/hour
 
  • BD FACS Canto A €20/hour
 
  • BD FACS Canto II €30/hour
 
  • BD FACS AriaII €60/hour + €100 setup

  • Biorad Bioplex 200 €100 per booking

Services Available
 
Services 
 
  • Access to high end, BD FACS Aria II, high speed cell sorter
 
  • Access to three analytical flow cytometers: BD FACS Canto A, BD FACS Canto II and BD Accuri C6 and two data analysis PC workstations
 
  • Access to Biorad Bioplex 200 multiplex array platform 
 
  • Provision of well maintained equipment subject to daily, weekly and monthly quality control checks
 
  • Provision of technical consultation and support regrading experimental design, sample preparation, data analysis and interpretation
 
  • Structured new facility user induction program and continued application support
 
  • Access to local, national and international flow cytometry facility networks




Training
 

 
 
Training

All Flow Cytometry Facility users are required to attend induction training sessions. Training sessions take place on Monday afternoons, 2pm to 5pm, in the Flow Cytometry Facility, Second Floor South, Biosciences. There are two types of training sessions offered, a training session specific for the Canto cytometers and a training session specific for the Accuri C6 sampler cytometer.


Places are limited to 4 people per session, the minimum number is 2. Please note the induction training session should be attended by those users who plan to use the flow cytometry facility within a short period of time; 'refresher' training is not offered.


If you would like to attend an induction training session, please e-mail: Shirley.Hanley@nuigalway.ie and state which induction training you require i.e. Canto or Accuri training. Please note, you will be required to perform online training modules before attending the induction training session and you must bring along 1 or 2 samples of cells (stained or unstained) to the training session.
 
The induction training session will cover a general introduction to flow cytometry, an overview of the cytometer and software and general flow cytometry facility housekeeping rules. The training sessions are not application specific. If a new user requires training on a specific application then a one to one training session can be scheduled with Dr. Hanley after the initial induction training.
 
Please note, keycard access and equipment booking access will only be given to those who have successfully completed the induction training sessions.



Publications

Flow Cytometry Facility Acknowledgement

Any formal presentations or publications resulting from work performed in the facility should be acknowledged with the following wording:

 ?The authors acknowledge the facilities and technical assistance of the Flow Cytometry Core Facility at NUI Galway, a facility that is funded by NUI Galway and the Irish Government's Programme for Research in Third Level Institutions, Cycle5, National Development Plan 2007-2013?

If considerable input into experimental design, sorting of samples, and/or analysis of results has been provided then co-authorship should be considered on subsequent publications.

 
 
 
 
Duffy MM, McNicholas BA, Monaghan DA, Hanley SA, McMahon JM, Pindjakova J, Alagesan S, Fearnhead HO, Griffin MD. Am J Physiol Renal Physiol. 2014 Dec 15;307(12):F1412-26. doi: 10.1152/ajprenal.00024.2014.
 
 
The influence of hypoxia on the differentiation capacities and immunosuppressive properties of clonal mouse mesenchymal stromal cell lines.
Prado-Lòpez S, Duffy MM, Baustian C, Alagesan S, Hanley SA, Stocca A, Griffin MD, Ceredig R. Immunol Cell Biol. 2014 Aug;92(7):612-23. doi: 10.1038/icb.2014.30.
 
 
Gerlach JQ, Krüger A, Gallogly S, Hanley SA, Hogan MC, Ward CJ, Joshi L, Griffin MD. PLoS One. 2013 Sep 19;8(9):e74801. doi: 10.1371/journal.pone.0074801.


Pindjakova J, Hanley SA, Duffy MM, Sutton CE, Weidhofer GA, Miller MN, Nath KA, Mills KH, Ceredig R, Griffin MD. Kidney Int. 2012 Feb;81(4):379-90. doi: 10.1038/ki.2011.348.
 
 
Duffy MM, Pindjakova J, Hanley SA, McCarthy C, Weidhofer GA, Sweeney EM, English K, Shaw G, Murphy JM, Barry FP, Mahon BP, Belton O, Ceredig R, Griffin MD. Eur J Immunology. 2011 Oct;41(10):2840-51. doi: 10.1002/eji.201141499.
 
 
 
 Developing cell-specific antibodies to endothelial progenitor cells using avian immune phage display technology.
Bowes T, Hanley SA, Liew A, Eglon M, Mashayekhi K, O'Kennedy R, Barry F, Taylor WR, O'Brien T, Griffin MD, Finlay WJ, Greiser U. J Biomol Screen. 2011 Aug;16(7):744-54. doi: 10.1177/1087057111407067.

 
 
Useful Links
 
Useful Links
 
Flow Cytometry Societies
 
Flow Cytometry UK
 
European Cytometry Network
 
International Society for the Advancement of Cytometry
 
 
Useful Web Tools
 
A free impartial resource for flow cytometry products, knowledge and services
 
BD Biosciences Flow Cytometry Training & e-Learning
 
BD Biosciences Multicolour Flow Tools
 
Life Technologies Flow Cytometry Resource Centre 
 
 
 
Useful Resources

Free online copy of Practical Cytometry
 
Purdue University International Flow Cytometry Mailing list
 
MIFlowCyt 1.0: Minimum Information about a Flow Cytometry Experiment
 
 
 
Other Flow Cytometry Facility Websites
 
School of Biochemistry and Immunology, Trinity College Dublin
 
University College Dublin

 
 
Contact Information
 
Contact Details
 
Dr Shirley Hanley
Flow Cytometry Facility Manager

Email: shirley.hanley@nuigalway.ie
Telephone: + 353 91 495825
 
 
Professor Rhodri Ceredig
Flow Cytometry Director
Director of the National Centre for Biomedical Engineering & Science (NCBES)
 
Email: rhodri.ceredig@nuigalway.ie
Telephone: + 353 91 495916
 
 
Facility Location
 
The flow Cytometry Core Facility is located on the Second Floor South of the Biosciences Building in NUI Galways north campus.