Core Facilities - BD FACSCanto A
Booking: To avoid block booking, users can book a maximum of 2 hour time slots between the hours of 9am and 4pm Monday to Friday. > 2 hour time slots can only be booked after 4pm Monday to Friday, or at weekends.
Circumstances where users require > 2 hour time slot during normal working hours may be accommodated provided that a minimum notice of 2 weeks is given to Dr Shirley Hanley to allow notification to all Canto users.
Usage: Only users who have completed the Canto A induction session can use the Canto A. New or infrequent users can only use the Canto during normal working hours, Mon - Fri 9am to 5pm and under the supervision of a more experienced user.
Induction: Induction training on how to correctly use the Canto and basic introduction to Flow Cytometry take place on Mondays from 11am to 1pm. Places are limited to 4 people, please e-mail email@example.com to book a place.
Flow cytometry is a qualitative and quantitative analysis technology that can characterise cell populations at a single cell level. Flow cytometry is a multi-parametric technology that measures fluorescent signals from the scattered light produced by cells as they pass or flow through a laser light source. These signals can becorrelated to structural and/or functional cell parameters.
Cellular features measurable by Flow Cytometry:
Intrinsic : size, shape, cytoplasmic granularity, autofluorescence and pigmentation
Extrinsic : DNA content, DNA composition, DNA synthesis, cell cycle, chromatin structure, RNA, protein, sulphydryl groups, Antigens (surface, cytoplasmic & nuclear), lectin binding sites, cytoskeletal components, membrane structure (potential, permeability & fluidity), enzyme activity, endocytosis, surface charge, receptors, bound and free calcium, apoptosis, necrosis, pH, drug kinetics, etc.