Core Facilities - BD FACSCanto A

Location: Flow Cytometry Facility, BB2-2044, Second Floor South, Bioscience Building.
Person responsible: Shirley Hanley
Email: shirley.hanley@nuigalway.ie
Phone: 091 495825
Canto A charges 2017: €10 per 30 mins or portion thereof. 1 hour minimum cancellation period.



Booking: To avoid block booking, users can book a maximum of 2 hour time slots between the hours of 9am and 4pm Monday to Friday. > 2 hour time slots can only be booked after 4pm Monday to Friday, or at weekends.

Circumstances where users require > 2 hour time slot during normal working hours may be accommodated provided that a minimum notice of 2 weeks is given to Dr Shirley Hanley to allow notification to all Canto users.



Usage: Only users who have completed the Canto A induction session can use the Canto A. New or infrequent users can only use the Canto during normal working hours, Mon - Fri 9am to 5pm and under the supervision of a more experienced user.





Induction: Induction training on how to correctly use the Canto and basic introduction to Flow Cytometry take place on Mondays from 11am to 1pm. Places are limited to 4 people, please e-mail shirley.hanley@nuigalway.ie to book a place.







Flow cytometry is a qualitative and quantitative analysis technology that can characterise cell populations at a single cell level. Flow cytometry is a multi-parametric technology that measures fluorescent signals from the scattered light produced by cells as they pass or flow through a laser light source. These signals can becorrelated to structural and/or functional cell parameters.



Cellular features measurable by Flow Cytometry:



Intrinsic : size, shape, cytoplasmic granularity, autofluorescence and pigmentation


 


Extrinsic : DNA content, DNA composition, DNA synthesis, cell cycle, chromatin structure, RNA, protein, sulphydryl groups, Antigens (surface, cytoplasmic & nuclear), lectin binding sites, cytoskeletal components, membrane structure (potential, permeability & fluidity), enzyme activity, endocytosis, surface charge, receptors, bound and free calcium, apoptosis, necrosis, pH, drug kinetics, etc.


BD FACS CANTO



The BD FACS Canto is capable of detecting up to 6 fluorescent parameters and 2 physical parameter per sample. The Canto can process up to 10,000 cells per second.

 

BD FACS CANTO 6 Colour Configuration:

 

Blue 488nm laser:

Filter                 Primary Flourochrome           Other Flourochromes


530/30nm          FITC                                      GFP, AF488


585/42nm          PE                                          PI


670nm LP           PerCP                                    PerCP Cy5.5, Pe-Cy5.5, PI

780/60               PE-Cy7                                   





Red 633nm laser:


 660/20                APC                                      AF647

780/60                 APC-Cy7                              


 

Useful links:


Introduction to flow cytometry and Diva software: http://www.bdbiosciences.com/support/training/self_paced.jsp


Multicolour spectral viewer: http://www.bdbiosciences.com/external_files/media/spectrumviewer/index.jsp

Multicolour flow cytometry:

http://www.bdbiosciences.com/eu/research/multicolor/index.jsp
 

 


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